This study investigated the antibacterial properties and mechanism of action of Paullinia pinnata extracts on wound isolates; determined the rate of killing with the view to harnessing it as base for synthesis of novel antimicrobial agent. The leaf of P. pinnata was extracted in ethanol for four days with regular agitation. The filtrate was concentrated using rotary evaporator at 40°C and fractionated on column chromatography. The extracts were assayed on the test organisms by agar diffusion technique. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts were carried out by agar dilution and agar diffusion techniques, respectively. The rate of kill-assay of ethyl acetate and ampicillin against Staphylococcus aureus and Proteus vulgaris was determined. The results showed that antibacterial activity of crude ethanolic extract of the leaf of P. pinnata varied greatly with the wound organisms. The crude extract at 35 mg/mL had the highest zone of inhibition (22.00 mm) against Staphylococcus spp (C1). The MIC and MBC of the crude extract were 2.19 and 17.50 mg/mL, respectively on the wound bacteria. The study concluded that the leaf extract of P. pinnata were found to possess antibacterial activity which compared positively with ampicillin.
Olatujoye F, Oluduro AO, Omololu-Aso J and Otusanya OO
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