BackgroundAim: Τhe investigation of the frequency of the burnout syndrome among the nursing personnel of all rungs in a Regional University Hospital.Methods: Τhe research was conducted in a Regional University Hospital. The questionnaire was distributed to 150 Registered Nurses (RN) and Nurses Assistants (NA) and the response rate was 42,6% (N=64). MBI questionnaire was used in order to identify levels of burnout syndrome. The analysis was made with the use of SPSS (version 15). Student t-test, ANOVA one way, chi-square (χ2) was performed.Results: Generally occupational burnout appears to be in moderate levels. 9,37% of the sample experienced a high degree of burnout while 6,24% experienced a low one. Emotional exhaustion correlates significantly with working a rotation shift (p=0,05). Emotional exhaustion correlates significantly with resignation from hospital (p=0,002). Depersonalization correlates significantly with the multidisciplinary cooperation (p=0,05).Conclusions: Environmental factors such as shift work, multidisciplinary cooperation are shown to be related with the appearance of the syndrome.